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A rapid DEAE disk assay for photoreactivation of pyrimidine dimers in [3H]DNA

✍ Scribed by William H. Farland; Betsy M. Sutherland

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
462 KB
Volume
97
Category
Article
ISSN
0003-2697

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✦ Synopsis

We have developed a rapid, sensitive, and specific assay for photoreactivation of pyrimidine dimers in 3H-labeled DNA. It is based on the nuclease resistance of dimercontaining sequences in DNA, and the adsorption of these sequences to DEAE-substituted paper (DE-81). The method maintains the advantages of the rapid dimer assay of B. M. Sutherland and M. J. Chamberlin (1973, Anal. Biochem., 53, 168-176), while avoiding its major drawback, the frequent preparation of high specific activity, 32P-labeled, bacteriophage DNA. In addition. the use of scintillation spectrometry in our assay should allow more widespread use of this method.

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