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A rapid culture-harvest protocol for amniotic cell cultures

โœ Scribed by Barathur R. Rajendra; Leonard J. Sciorra; Ming-liang Lee


Publisher
Springer
Year
1981
Tongue
English
Weight
629 KB
Volume
59
Category
Article
ISSN
0340-6717

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A time proven technique is described for growing amniotic fluid cell cultures on cover glasses in Leighton tubes and for processing the mitotic cells in situ. Karyotyping the clones in situ eliminates most of the problems caused by somatic chromosome mutations in vitro and by maternal cell growth.