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A rapid column chromatographic method for the isolation of catechol-type siderophores

โœ Scribed by A.V. Robinson

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
551 KB
Volume
95
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A column chromatographic method using a polyamide support has been developed which allows rapid isolation of catechol-type siderophores directly from culture supematants. The method is superior to presently used methods with regard to speed, convenience, and lack of degradation products. Siderophores from Micrococcus denitrifcans (ATCC 19367), Klebsiella nerogenes (ATCC 13882), and Bacillus subtilis (ATCC 6051) have been purified and the method has been tested with two synthetic catechol-type chelators, Nr,N*-bis(2,3dihydroxybenzoyl)ethylenediamine and N',N'-bis(2,3-dihydroxybenzoyl)putrescine.

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