A rapid colorimetric method for oestriol assay in pregnancy urine
β Scribed by M. Rahman
- Book ID
- 115819257
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 326 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0009-8981
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π SIMILAR VOLUMES
A batch resin method for determination of 3-methylhistidine is described. Selectivity of the resin and the specificity of the color reagent combine to eliminate the interference of other amino acids. Lyophilizing the urine sample with sodium carbonate removes the ammonia interference.
A method is described for the measurement of oestriol in pregnancy urine. After hydrolysis of the urine, oestrogens are reacted with r-dimethylaminonaphthalene-5sulphonyl chloride (DANSYL-chloride) to give fluorescent derivatives. The oestriol derivative is separated by thin-layer chromatography on