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A rapid colorimetric assay for γ-glutamyl hydrolase (conjugase)

✍ Scribed by Premanand V. Wagh; Thomas I. Kalman


Book ID
107719088
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
594 KB
Volume
207
Category
Article
ISSN
0003-2697

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A simple two-step method is described for quantitating the release of free L-phenylalanine, L-leucine, L-methionine, or L-isoleucine from di-or polypeptides. The calorimetric assay is based on the ability of L-amino acid oxidase to catalyze the oxidation of free L-amino acid, but not of peptides. Th