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A colorimetric assay for S-adenosylhomocysteine hydrolase

✍ Scribed by J.D. Lozada-Ramírez; I. Martínez-Martínez; A. Sánchez-Ferrer; F. García-Carmona

Book ID
113681770
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
170 KB
Volume
67
Category
Article
ISSN
0165-022X

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A simple two-step method is described for quantitating the release of free L-phenylalanine, L-leucine, L-methionine, or L-isoleucine from di-or polypeptides. The calorimetric assay is based on the ability of L-amino acid oxidase to catalyze the oxidation of free L-amino acid, but not of peptides. Th