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A rapid assay to measure collagen synthesis in cell cultures

โœ Scribed by Markku Jalkanen; Markku Tammi; Timo Vihersaari; Juha Peltonen; Eino Kulonen; Risto Penttinen

Book ID
119506272
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
622 KB
Volume
2
Category
Article
ISSN
0165-022X

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โœ R.F. Diegelmann; G.R. Bryson; L.C. Flood; M.F. Graham ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 627 KB

A method to quantitate collagen synthesis, total protein synthesis, and DNA in 24-well culture plates is presented. Collagen-producing cells such as human intestinal smooth muscle cells and dermal fibroblasts were pulse-labeled with [3H]proline. After incubation, the plates were heated to 90 degrees