A rapid assay for mitochondrial DNA damage and respiratory chain inhibition in the yeast Saccharomyces cerevisiae
โ Scribed by Barry J. Barclay; Carrie L. DeHaan; Ursula G.G. Hennig; Oksana Iavorovska; Reid W. von Borstel; R.C. von Borstel
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 313 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0893-6692
- DOI
- 10.1002/em.1066
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Molecular analysis of mitochondrial DNA (mtDNA) is a critical step in diagnosis and genetic counseling of respiratory chain defects. No fast method is currently available for the identification of unknown mtDNA point mutations. We have developed a new strategy based on complete mtDNA PCR amplificati
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