A rapid assay for cellular deoxyribonucleic acid

✍ Scribed by J.Edward Puzas; David B.P. Goodman

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 330 KB
• Volume: 86
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90317-2

✦ Synopsis

A method is described for the rapid and specific extraction and subsequent fluorometric assay of DNA from less than 2 x lo4 diploid mammalian cells-O. 1 pg of DNA. The procedure can be routinely completed in 3 hr and can be applied to quantitation of [3H]thymidine incorporation into these small quantities of DNA.

Methods

Purified calf thymus DNA or bone cells isolated from fetal rat calvaria (11) were used as sources of DNA. The cells were either in suspension or in