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A rapid and ultrasensitive method for measurement of DNA, calcium and protein content, and alkaline phosphatase activity of chondrocyte cultures

โœ Scribed by C. C. Teixeira; M. Hatori; P. S. Leboy; M. Pacifici; I. M. Shapiro


Publisher
Springer
Year
1995
Tongue
English
Weight
531 KB
Volume
56
Category
Article
ISSN
1432-0827

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A simple, rapid, and sensitive method for the measurement of DNA, RNA, and protein synthesis in cell samples is presented. Tke method measures the amount of isotope incorporated into these macromolecules after cell collection on filter paper and washing with a methanol:chloroform:water mixture. Samp