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A rapid and specific assay for Nα-acetyltransferase activity

✍ Scribed by H.P.C. Driessen; W.W. de Jong; G.I. Tesser; H. Bloemendal

Book ID
118308734
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
434 KB
Volume
125
Category
Article
ISSN
0003-2697

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Dopamine (DA) was measured in various tissue extracts as [3H]methoxy-N-acetyldopamine after incubation with two partially purified enzymes, catechol-O-methyl transferase (EC 2.1.1.1) and N-acetyltransferase (EC 2.3.1.5), in the presence of [3H]adenosylmethionine and acetyl-CoA. This product can be s