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A rapid and efficient approach to metabolite identification using nuclear magnetic resonance spectroscopy, liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance spectroscopy/sequential mass spectrometry

โœ Scribed by G. J. Dear; J. Ayrton; R. Plumb; B. C. Sweatman; I. M. Ismail; I. J. Fraser; P. J. Mutch

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
118 KB
Volume
12
Category
Article
ISSN
0951-4198

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โœฆ Synopsis

High-performance liquid chromatography, nuclear magnetic resonance (NMR) spectroscopy and sequential mass spectrometry (MS n ) have been combined to allow rapid and effective identification of metabolites in urine following administration of the novel non-nucleoside reverse transcriptase inhibitor, GW420867. The combination of these techniques has enabled structural and molecular weight information to be interpreted by the analyst with greater efficiency and accuracy. This work demonstrates the potential of coupling MS n using an ion trap spectrometer with NMR spectroscopy, providing an extremely powerful technique for structural elucidation.

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