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A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

✍ Scribed by Kathleen M. Knights; Roger Drew

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
476 KB
Volume
168
Category
Article
ISSN
0003-2697

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✦ Synopsis

A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

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