Assay of Coenzyme Q10 in Plasma by a Single Dilution Step

✍ Scribed by Fabrizio Mosca; Daniele Fattorini; Stefano Bompadre; Gian Paolo Littarru

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 74 KB
• Volume: 305
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2002.5653

✦ Synopsis

A new method is described for determining coenzyme Q 10 (CoQ 10 ) in plasma. The method is based on oxidation of CoQ 10 in the sample by treating it with para-benzoquinone followed by extraction with 1-propanol and direct injection into the HPLC apparatus. This method achieves a linear detector response for peak area measurements over the concentration range of 0.05-3.47 M. Diode array analysis of the peak was consistent with CoQ 10 spectrum. Supplementation of the samples with known amounts of CoQ 10 yielded a quantitative recovery of 96 -98.5%; the method showed a level of quantitation of 1.23 nmol per HPLC injection (200 l of propanol extract containing 33.3 l of plasma). A correlation of r ‫؍‬ 0.99 (P < 0.0001) was found with a reference electrochemical detection method. Within run precision showed a CV% of 1.6 for samples approaching normal values (1.02 M). Day-today precision was also close to 2%.