✦ LIBER ✦

A radioactive assay for acetylcholinesterase using anion-exchange disk

✍ Scribed by Richard K. Gordon; B.P. Doctor; Peter K. Chiang

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 321 KB
Volume: 124
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90048-3

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✦ Synopsis

A radioactive assay for acetylcholinesterase is described. The assay is based on the separation of [%]acetate from ["C]acetylcholine by differential adsorption of the former on DEAE anion-exchange disks. The procedure is simple and sensitive and eliminates the use of ionexchange resin columns or organic extractions. Moreover, when unpurified enzyme preparations are assayed, linear steady-state kinetics can be observed with this method as contrasted to the nonlinear calorimetric method using acetylthiocholine and dithiobisnitrobenzoate. This method also permits the detection in biological samples of low levels of acetylcholinesterase activity, which is not detectable by the calorimetric method. Using the present radioactive method, cellular levels of acetylcholinesterase have been surveyed in N4TGl neuroblastoma cells, NG108-15 neuroblastoma x glioma hybrid cells, H9c2 myoblasts, and 3T3-Ll and 3T3-C2 fibroblasts.

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