A Radioactive Assay for Sialyltransferase Activity Using 96-Well Multiscreen Filtration Plates
โ Scribed by Wouter Laroy; Marleen Maras; Walter Fiers; Roland Contreras
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 108 KB
- Volume
- 249
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
We describe two alternative assays for measuring collagenolytic activity using 3 H-acetylated collagen. Both assays have been developed for the 96-well plate format and measure the amount of radiolabeled collagen fragments released into the supernatant from an insoluble 3 H-acetylated collagen fibri
ers such as piperazine diacrylamide in place of bisacrylamide. In general, if gel conditions other than those described in this paper are to be employed, an experiment analogous to that shown in Fig. 2a should be carried out to determine the optimal PEG concentration for those specific conditions.