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A Quick Method for Cholesterol Titration in Complex Media

✍ Scribed by Pioch, D. ;Lozano, P. ;Frater, C. ;Graille, J.

Publisher
John Wiley and Sons
Year
1992
Weight
493 KB
Volume
94
Category
Article
ISSN
0931-5985

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We offer a new titration protocol for determining the dissociation constant and binding stoichiometry of protein-ligand complex, detectable by spectroscopic methods. This approach neither is limited to the range of protein or ligand concentrations employed during titration experiment nor relies on p