✦ LIBER ✦

A Quantitative Polymerase Chain Reaction Assay for Interleukin 5 Messenger RNA

✍ Scribed by A. Guiffre; K. Atkinson; P. Kearney

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 724 KB
Volume: 212
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1290

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Quantitative plasmid mixture analysis us

Quantitative plasmid mixture analysis using the fluorogenic 5′-nuclease polymerase chain reaction assay

✍ Alison E. Director-Myska; Wendy K. Pogozelski; Richard S. Lofts; Pataje G.S. Pra 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB

Quantitative Polymerase Chain Reaction w

Quantitative Polymerase Chain Reaction with Oligodeoxynucleotide Ligation Assay/Enzyme-Linked Immunosorbent Assay Detection

✍ P. Friedhoff; M. Hahn; H. Wolfes; A. Pingoud 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 743 KB

A flexible bioluminescent-quantitative p

A flexible bioluminescent-quantitative polymerase chain reaction assay for analysis of competitive PCR amplicons

✍ Jeffrey K. Actor; Josef R. Limor; Robert L. Hunter 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB

Aequorin-based flash-type bioluminescent methods can detect nucleic acid molecules in the attomolar range (10(-18)) enabling improved monitoring of the polymerase chain reaction (PCR) at cycles previously considered too low for product detection. The high sensitivity of bioluminescence (BL) was used

Homogeneous Quantitative Assay of Hepati

Homogeneous Quantitative Assay of Hepatitis C Virus RNA by Polymerase Chain Reaction in the Presence of a Fluorescent Intercalater

✍ T. Ishiguro; J. Saitoh; H. Yawata; H. Yamagishi; S. Iwasaki; Y. Mitoma 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 564 KB

We have developed a homogeneous quantitative assay of DNA/RNA by performing PCR in the presence of an oxazole yellow derivative, a fluorescent DNA intercalative dye, and monitoring the fluorescence intensity of the PCR reaction mixture during PCR cycles. We have demonstrated the applicability of thi

One-Step Duplex Reverse Transcription–Po

One-Step Duplex Reverse Transcription–Polymerase Chain Reaction for Quantitative Assessment of RNA Degradation

✍ Michio Sugita; Jerry L. Haney; Robert M. Gemmill; Wilbur A. Franklin 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 78 KB

A New Quantitative Method of Real Time R

A New Quantitative Method of Real Time Reverse Transcription Polymerase Chain Reaction Assay Based on Simulation of Polymerase Chain Reaction Kinetics

✍ Weihong Liu; David A. Saint 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 108 KB

Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference