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A quantitative method for the determination of the activities of mushroom tyrosinase

✍ Scribed by M.H. Keyes; F.E. Semersky

Book ID
115701312
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
428 KB
Volume
148
Category
Article
ISSN
0003-9861

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A sensitive, rapid, quantitative method for the determination of the activities of the bifunctional frog epidermis enzyme, tyrosinase (E.C. 1.14.18. l), has been developed. It is a spectrophotometric method using p-coumaric acid and caffeic acid as substrates at pH 7.0. Lineweaver-Burk plots yielded