A new spectrophotometric method for the determination of cresolase activity of epidermis tyrosinase

✍ Scribed by F.G. Carmona; E. Pedreño; J.D. Galindo; F.G. Cánovas

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 220 KB
• Volume: 95
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90751-6

✦ Synopsis

A sensitive, rapid, quantitative method for the determination of the activities of the bifunctional frog epidermis enzyme, tyrosinase (E.C. 1.14.18. l), has been developed. It is a spectrophotometric method using p-coumaric acid and caffeic acid as substrates at pH 7.0. Lineweaver-Burk plots yielded straight lines and the initial velocities for the reactions were proportional to enzyme concentrations. It is simpler, faster, and more economical than radiometric methods and, furthermore, permits continuous monitoring.