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A Pyrrolysine Analogue for Protein Click Chemistry

✍ Scribed by Tomasz Fekner; Xin Li; Marianne M. Lee; Michael K. Chan

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 423 KB
Volume: 121
Category: Article
ISSN: 0044-8249
DOI: 10.1002/ange.200805420

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✦ Synopsis

Abstract

Mithilfe des UAG‐Codons wurde ein Pyrrolysin‐Analogon mit terminaler Alkinfunktion ortsspezifisch in rekombinantes Calmodulin (CaM) eingebaut. Das resultierende Protein wurde über eine Kupfer(I)‐katalysierte Klick‐Reaktion mit der Azidgruppe eines Farbstoffs verknüpft. Die anschließende Anwendung einer orthogonalen Cystein‐Markierungsmethode ergab mit zwei unterschiedlichen Fluorophoren markiertes CaM für FRET‐Studien.magnified image

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