High-Density Glycosylation of Polymer Membrane Surfaces by Click Chemistry for Carbohydrate–Protein Recognition
✍ Scribed by Cang Wang; Jian Wu; Zhi-Kang Xu
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 302 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1022-1336
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✦ Synopsis
Abstract
To biologically mimic the carbohydrate–protein interactions in artificial systems, one of the challenges is to construct a glycosylated surface with a high glycosyl density to yield a notable ‘glycoside cluster effect’. A novel strategy is presented for high density glycosylation of the surface of a microporous poly(propylene) membrane (MPPM) by click chemistry. It is promising that the surface glycosyl density can be well controlled over a wide range and the maximum value is over 10 µmol · cm^−2^. The recognition capability of these glycosylated MPPMs to lectins indicates the occurrence of the ‘glycoside cluster effect’ when the glycosyl density on the membrane surface exceeds 0.20 µmol · cm^−2^.
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