A Protein Biosensor for Lactate
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Sabato D'Auria; Zygmunt Gryczynski; Ignacy Gryczynski; Mosรฉ Rossi; Joseph R. Lak
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Article
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2000
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Elsevier Science
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English
โ 98 KB
Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an appr