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A process for bioconversion of cephalosporin C byRhodotorula gracilisD-amino acid oxidase

✍ Scribed by Mirella S. Pilone; Simona Butò; Loredano Pollegioni

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
393 KB
Volume
17
Category
Article
ISSN
0141-5492

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✦ Synopsis

A process for the production (in a stirred tank reactor) of glutaryl-7-ACA from cephalosporin C using immobilized D-amino acid oxidase is described. Results so obtained under optimal conditions (1.2 mg coupled enzyme/L, pH 8.5, 2 mM cephalosporin C) point to a system which shows high conversion efficiency and a remarkable operational stability. No exogenous H202 is requested to shift the reaction equilibrium toward glutaryl-7-ACA production, nor any side product is detected. The immobilized system productivity was 54 g/day/mg of enzyme. This process represents the first reported case of a reactor successfully developed with a DAAO for bioconversion of cephalosporin C.

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