A simple, rapid, and sensitive procedure
โ
M. Saleemuddin; Hassan Ahmad; Athar Husain
๐
Article
๐
1980
๐
Elsevier Science
๐
English
โ 350 KB
A rapid calorimetric method for the assay of proteolytic enzymes based on the binding of Coomassie brilliant blue G-250 to unhydrolyzed protein substrate is described. Considerable assay time is saved since the method does not require the separation of the hydrolyzed products from the undegraded pro