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A procedure to increase the sensitivity of staining by Coomassie brilliant blue G250-perchloric acid solution

โœ Scribed by I.B. Holbrook; A.G. Leaver


Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
599 KB
Volume
75
Category
Article
ISSN
0003-2697

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A simple, rapid, and sensitive procedure
โœ M. Saleemuddin; Hassan Ahmad; Athar Husain ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 350 KB

A rapid calorimetric method for the assay of proteolytic enzymes based on the binding of Coomassie brilliant blue G-250 to unhydrolyzed protein substrate is described. Considerable assay time is saved since the method does not require the separation of the hydrolyzed products from the undegraded pro