A procedure for the preferential radioiodination of small amounts of protein in the presence of excess lipids

✍ Scribed by Ronald C. Montelaro; Dani P. Bolognesi

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 365 KB
• Volume: 99
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90047-2

✦ Synopsis

The chloramine-T, iodine monochloride, and lactoperoxidase radioiodination procedures were evaluated for their ability to label proteins in the presence of large amounts of reactive lipid. Mouse serum very low density lipoproteins (VLDL) (10% protein, 90% lipid) and detergent-disrupted Friend murine leukemia virus (FLV) (60% protein, 33% lipid) were used as model systems. Based on the distribution of label between protein and lipid, as well as the total amount of label incorporated by each procedure, only a modification of the iodine monochloride procedure preferentially labeled proteins to a high specific activity in both VLDL and FLV. The technique, which is described in detail, is technically simple, rapid, and causes no detectable degradation of proteins.