A Practical Guide to Membrane Protein Pu
โ Gebhard von Jagow and Arnold Revzin (Eds.)
๐ Library
๐
1994
๐ English
โฆ LIBER โฆ
๐
A Practical Guide to Protein Engineering
โ Scribed by Tuck Seng Wong, Kang Lan Tee
- Publisher
- Springer International Publishing;Springer
- Year
- 2020
- Tongue
- English
- Leaves
- 213
- Series
- Learning Materials in Biosciences
- Edition
- 1st ed.
- Category
- Library
โฌ Acquire This Volume
No coin nor oath required. For personal study only.
โฆ Synopsis
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
โฆ Table of Contents
Front Matter ....Pages I-XV
Literature Search (Tuck Seng Wong, Kang Lan Tee)....Pages 1-10
Sequence Analysis (Tuck Seng Wong, Kang Lan Tee)....Pages 11-27
Structural Analysis (Tuck Seng Wong, Kang Lan Tee)....Pages 29-38
Protein Expression Hosts and Expression Plasmids (Tuck Seng Wong, Kang Lan Tee)....Pages 39-61
Gene Cloning (Tuck Seng Wong, Kang Lan Tee)....Pages 63-86
Protein Expression (Tuck Seng Wong, Kang Lan Tee)....Pages 87-92
Assay (Tuck Seng Wong, Kang Lan Tee)....Pages 93-119
Gene Mutagenesis (Tuck Seng Wong, Kang Lan Tee)....Pages 121-148
High-Throughput Screening (HTS) (Tuck Seng Wong, Kang Lan Tee)....Pages 149-168
Protein Variants Analysis and Characterization (Tuck Seng Wong, Kang Lan Tee)....Pages 169-186
Continuing from Protein Variants (Tuck Seng Wong, Kang Lan Tee)....Pages 187-195
Employability (Tuck Seng Wong, Kang Lan Tee)....Pages 197-202
โฆ Subjects
Life Sciences; Protein Science; Biochemical Engineering; Biological Techniques
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