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A Practical Guide to Membrane Protein Purification

✍ Scribed by Gebhard von Jagow and Arnold Revzin (Eds.)


Year
1994
Tongue
English
Leaves
166
Category
Library

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✦ Table of Contents


Content:
Separation, Detection, and Characterization of Biological Macromolecules, Page ii
Front Matter, Page iii
Copyright, Page iv
Contributors, Pages xi-xii
Preface to the Series, Pages xiii-xiv, Arnold Revzin
Preface to the Volume, Pages xv-xvi, Gebhard von Jagow, Hermann SchΓ€gger
Abbreviations, Page xvii
1 - Purification Strategies for Membrane Proteins, Pages 3-21, GEBHARD VON JAGOW, THOMAS A. LINK, HERMANN SCHΓ„GGER
2 - Chromatographic Techniques and Basic Operations in Membrane Protein Purification, Pages 23-57, HERMANN SCHΓ„GGER
3 - Denaturing Electrophoretic Techniques, Pages 59-79, HERMANN SCHΓ„GGER
4 - Native Gel Electrophoresis, Pages 81-104, HERMANN SCHΓ„GGER
5 - Isolation of Complex III, Subcomplexes, and Protein Subunits from Bovine Heart: Triton X-100/Hydroxylapatite, Pages 107-124, HERMANN SCHΓ„GGER
6 - Chromatographic Purification of F1F0-ATPase and Complex I from Bovine Heart Mitochondria: FPLC-Anion Exchange and Dye-Ligand Chromatography, Pages 125-133, SUSAN K. BUCHANAN, JOHN E. WALKER
7 - Isolation of Ubiquinol: Cytochrome c Oxidoreductase (Complex III) from Fungal and Plant Mitochondria: Affinity Chromatography, Pages 135-140, MARTIN BΓ–DICKER, HANNS WEISS
8 - Isolation of Wolinella succinogenes Hydrogenase Chromatofocusing, Pages 141-147, ACHIM KRΓ–GER, VIKTOR GEISLER, ANDREA DUCHĚNE
9 - Photosystem I from Cyanobacteria Isolated in Crystallizing Form by Preparative Isoelectric Focusing: Isoelectric Focusing, Pages 149-159, GEORGIOS TSIOTIS
Index, Pages 161-166


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