๐”– Scriptorium
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๐Ÿ“

A Practical Guide to Protein and Peptide Purification for Microsequencing

โœ Scribed by Paul T. Matsudaira (Eds.)


Publisher
Academic Press
Year
1993
Tongue
English
Leaves
205
Edition
2
Category
Library

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โœฆ Synopsis


Why a Second Edition?
The Second Edition provides practical answers to the general question, ''How can I obtain useful sequence information from my protein or peptide?'' rather than the more specific question asked in the first edition, ''How can I obtain the N-terminal sequence?'' Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

Key Features
Highlights of the Second Edition:
Mass spectrophotometric characterization of proteins and peptides
N-terminal sequencing of proteins with blocked N termini
Internal amino acid sequence analysis after protease digestion in-gel and on-blot
Improved microscale peptide purification methods
Computer analysis of protein sequences
New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

โœฆ Table of Contents


Content:


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