Why a Second Edition?<br>The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new meth
A Practical Guide to Protein and Peptide Purification for Microsequencing
โ Scribed by Paul T. Matsudaira (Eds.)
- Publisher
- Academic Press
- Year
- 1993
- Tongue
- English
- Leaves
- 205
- Edition
- 2
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
Why a Second Edition?
The Second Edition provides practical answers to the general question, ''How can I obtain useful sequence information from my protein or peptide?'' rather than the more specific question asked in the first edition, ''How can I obtain the N-terminal sequence?'' Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Key Features
Highlights of the Second Edition:
Mass spectrophotometric characterization of proteins and peptides
N-terminal sequencing of proteins with blocked N termini
Internal amino acid sequence analysis after protease digestion in-gel and on-blot
Improved microscale peptide purification methods
Computer analysis of protein sequences
New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing
โฆ Table of Contents
Content:
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