𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A practical guide for solid-state NMR distance measurements in proteins

✍ Scribed by Frank A. Kovacs; Daniel J. Fowler; Gregory J. Gallagher; Lynmarie K. Thompson

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
679 KB
Volume
30A
Category
Article
ISSN
1546-6086

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Rotational resonance (R^2^) and rotational echo double resonance (REDOR) are powerful solid‐state NMR techniques that can be applied in a site‐directed fashion for precise distance measurements in proteins. These tools are well suited for systems in which a few precise distance measurements are needed to understand a mechanism or map a binding site, particularly if this information is unavailable from x‐ray crystallography or solution NMR, as is often the case for membrane proteins. Strategies and challenges in the design and implementation of such experiments are described and illustrated with experiments probing mechanisms of transmembrane signaling in bacterial chemotaxis receptors. Β© 2007 Wiley Periodicals, Inc. Concepts Magn Reson Part A 30A: 21–39, 2007.

πŸ“œ SIMILAR VOLUMES

The Accuracy of Distance Measurements in
The Accuracy of Distance Measurements in Solid-State NMR
✍ Paul Hodgkinson; Lyndon Emsley πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 517 KB

The accuracy with which distances can be measured using dipolar recoupling experiments in solid-state NMR is investigated. The relative precision of experiments in a three spin system versus an isolated spin pair is found to depend very strongly on the nature of the coupling Hamiltonian. The accurac

Practical Methods for Solid-State NMR Di
Practical Methods for Solid-State NMR Distance Measurements on Large Biomolecules: Constant-Time Rotational Resonance
✍ Yael S. Balazs; Lynmarie K. Thompson πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 72 KB

Simple modifications of the rotational resonance experiment substantially reduce the total experimental time needed to measure weak homonuclear dipolar couplings, a critical factor for achieving routine internuclear distance measurements in large biomolecular systems. These modifications also addres

Measurement of Internuclear Distances in
Measurement of Internuclear Distances in Solid-State NMR by a Background-Filtered REDOR Experiment
✍ Frederick G Vogt; Susan M Mattingly; James M Gibson; Karl T Mueller πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 106 KB

A background-filtered version of the rotational-echo double resonance (REDOR) experiment is demonstrated. The experiment combines a traditional REDOR pulse sequence with a double-cross-polarization (DCP) sequence to select only those signals coming from spin pairs of interest. The relatively ineffic