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A potential source of error in base-ratio analysis of ribonucleic acid prepared using bentonite

โœ Scribed by Hitchborn, J.H.; Dunn, D.B.

Book ID
122878192
Publisher
Elsevier Science
Year
1963
Weight
137 KB
Volume
68
Category
Article
ISSN
0926-6550

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Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with