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A potential procedure for obtaining mutants of Escherichia coli that affect plasmid stability

โœ Scribed by Iyer, V. N. ;Messmer, I.


Book ID
104691498
Publisher
Springer
Year
1974
Tongue
English
Weight
339 KB
Volume
133
Category
Article
ISSN
0026-8925

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A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth