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A postfermentative stage improves penicillin acylase production by a recombinantE. coli

✍ Scribed by Antonio León; Enrique Galindo; Octavio T. Ramírez

Book ID
104645896
Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
402 KB
Volume
18
Category
Article
ISSN
0141-5492

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✍ Isabel S. Novella; Claire Fargues; Georges GréVillot 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 396 KB 👁 2 views

Several techniques for protein extraction were tested for recovering penicillin acylase from a recombinant strain of Escherichia coli. These techniques include chemical [guanidine hydrochloride, Triton X-100, ethylenediaminetetraacetic acid (EDTA), ethanol/toluene], physical (sonication, freeze-and-