✦ LIBER ✦

A physiological role for DNA supercoiling in the anaerobic regulation of colicin gene expression

✍ Scribed by Malkhosyan, S. R. ;Panchenko, Yu. A. ;Rekesh, A. N.

Publisher: Springer
Year: 1991
Tongue: English
Weight: 653 KB
Volume: 225
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00269868

No coin nor oath required. For personal study only.

✦ Synopsis

The mechanism of anaerobic regulation of synthesis of colicins E1, E2, E3, K and D was studied. It was found that anaerobiosis significantly increases expression of the genes for colicins E1, E2, E3, K, and D. Experiments with novobiocin (a DNA gyrase inhibitor) showed that colicin synthesis in minicells and derepressed colicin synthesis in cells are dramatically reduced by relaxation of DNA supercoiling. A good correlation was observed between the levels of colicin synthesis and plasmid DNA supercoiling and the degree of aeration of the cultures. Thus, the regulation of colicin gene expression in response to a change in aeration appears to be mediated by environmentally induced variations in DNA supercoiling.

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