✦ LIBER ✦

A photoaffinity probe for 5-hydroxyeicosanoid dehydrogenase suitable for radioiodination

✍ Scribed by Seongjin Kim; Yurdanur Adiyaman; Goutam Saha; William S Powell; Joshua Rokach

Publisher: Elsevier Science
Year: 2001
Tongue: French
Weight: 83 KB
Volume: 42
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(01)00699-2

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✦ Synopsis

5-Hydroxy eicosanoid dehydrogenase (5h-dh) is a key enzyme responsible for the biosynthesis of 5-oxo-ETE, a potent eosinophil chemoattractant. To facilitate the identification and characterization of 5h-dh we have synthesized a photoaffinity ligand 7, designed to bind to the enzyme, and shown it to be an excellent substrate for 5h-dh. We have also synthesized a precursor 34, containing the photoaffinity label and a trimethyl tin group that can readily be displaced by iodide and will be suitable for radioiodination with 125 I.

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The design and synthesis of a 5-HETE affinity chromatography ligand for 5-hydroxyeicosanoid dehydrogenase

✍ Subhash P Khanapure; Goutam Saha; William S Powell; Joshua Rokach 📂 Article 📅 2000 🏛 Elsevier Science 🌐 French ⚖ 162 KB

The ®rst total synthesis of an o-amino 5-HETE derivative 27 has been accomplished by a new counterclockwise strategy, in which C-1 is constructed ®rst and C-20 last. The o-amino 5-HETE derivative was transformed to an anity chromatography ligand, the biotinylated 5-HETE 30. This anity chromatography