𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Perifusion System to Control Oxygen Concentration in Cell Suspensions

✍ Scribed by P.G. Arthur; C.T. Ngo; C.M. Wakeford

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
126 KB
Volume
263
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Measurement of the intracellular concent
Measurement of the intracellular concentration of oxygen in a cell perfusion system
✍ Kai Chen; Cheng E. Ng; Jay L. Zweier; Periannan Kuppusamy; Jerry D. Glickson; Ha πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 479 KB

## Abstract [O~2~] was measured in the embedding material (alginate) in a typical apparatus for conducting studies of viable cells with NMR, using low frequency EPR. In suspension cultures respiration was independent of [O~2~] in the perfusing media down to about 1 ΞΌM while in alginate beads, the c

Gel-entrapment of perfluorocarbons: A fl
Gel-entrapment of perfluorocarbons: A fluorine-19 NMR spectroscopic method for monitoring oxygen concentration in cell perfusion systems
✍ K. A. McGovern; J. S. Schoeniger; J. P. Wehrle; C. E. Ng; J. D. Glickson πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 844 KB

## Abstract Oxygenation is a major determinant of the physiological state of cultured cells. ^19^F NMR can be used to determine the oxygen concentration available to cells immobilized in a gel matrix by measuring the relaxation rate (1/T~1~) of perfluorocarbons (PFC) incorporated into the gel matri

The controlled presentation of TGF-Ξ²1 to
The controlled presentation of TGF-Ξ²1 to hepatocytes in a 3D-microfluidic cell culture system
✍ Chi Zhang; Ser-Mien Chia; Siew-Min Ong; Shufang Zhang; Yi-Chin Toh; Danny van No πŸ“‚ Article πŸ“… 2009 πŸ› Elsevier Science 🌐 English βš– 677 KB

3D-microfluidic cell culture systems (3D-microFCCSs) support hepatocyte functions in vitro which can be further enhanced by controlled presentation of 100-200 pg/ml TGF-beta1, thus mimicking the roles of supporting cells in co-cultures. Controlled presentation of TGF-beta1 is achieved by either dire

Time-resolved detection of singlet oxyge
Time-resolved detection of singlet oxygen luminescence in red-cell ghost suspensions: concerning a signal component that can be attributed to 1O2 luminescence from the inside of a native membrane
✍ Stefan Oelckers; Thomas Ziegler; Ingolf Michler; Beate RΓΆder πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 233 KB

For about ten years, it has been debated whether in principle it is possible to detect 1 O 2 located within the cell membrane by performing experiments with cell suspensions or even in tissue. In this paper we present our investigations on photosensitized red-cell ghost suspensions (RCGSs) and our s

p53 DOES NOT CONTROL THE SPINDLE ASSEMBL
p53 DOES NOT CONTROL THE SPINDLE ASSEMBLY CELL CYCLE CHECKPOINT BUT MEDIATES G1 ARREST IN RESPONSE TO DISRUPTION OF MICROTUBULE SYSTEM
✍ Anna A Sablina; Larissa S Agapova; Peter M Chumakov; Boris P Kopnin πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 318 KB

p53 plays a critical role as a tumour-suppressor in restricting the proliferation of damaged cells, thus preventing formation of genetically altered cell clones. Its inactivation leads, in particular, to accumulation of polyploid and aneuploid cells. To elucidate the role of p53 in control of chromo