Time-resolved detection of singlet oxygen luminescence in red-cell ghost suspensions: concerning a signal component that can be attributed to 1O2 luminescence from the inside of a native membrane
✍ Scribed by Stefan Oelckers; Thomas Ziegler; Ingolf Michler; Beate Röder
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 233 KB
- Volume
- 53
- Category
- Article
- ISSN
- 1011-1344
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✦ Synopsis
For about ten years, it has been debated whether in principle it is possible to detect 1 O 2 located within the cell membrane by performing experiments with cell suspensions or even in tissue. In this paper we present our investigations on photosensitized red-cell ghost suspensions (RCGSs) and our strategy for the detection of luminescence of singlet oxygen ( 1 O 2 ) from the inside of the cell membrane. Using a very sensitive apparatus for time-resolved 1 O 2 detection, a very promising sensitizer and an adequate experimental strategy, a very small amount of the detected luminescence indeed can be attributed to 1 O 2 from the inside of the ghost membrane.