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A peptide derived from pICln induced a strong hypotonic resistance in Escherichia coli cells☆

✍ Scribed by Guo-Zhong Tao; Yohtalou Tashima

Book ID: 117407746
Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 158 KB
Volume: 21
Category: Article
ISSN: 0196-9781
DOI: 10.1016/s0196-9781(00)00176-5

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led