Three native E. coli proteins-NusA, GrpE, and bacterioferritin (BFR)-were studied in fusion proteins expressed in E. coli for their ability to confer solubility on a target insoluble protein at the C-terminus of the fusion protein. These three proteins were chosen based on their favorable cytoplasmi
A parametric study of cloned fusion protein expression in Escherichia coli
✍ Scribed by J-H Seo; A. I. Löffler; J. E. Bailey
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 500 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0006-3592
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## Abstract The influence of linker design on fusion protein production and performance was evaluated when a family 9 carbohydrate‐binding module (CBM9) serves as the affinity tag for recombinant proteins expressed in __Escherichia coli__. Two bioinformatic strategies for linker design were applied