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A one-step biuret assay for protein in the presence of detergent

โœ Scribed by Christopher Watters

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
200 KB
Volume
88
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

An alkaline-copper reagent is described which forms a soluble, stable complex with protein in the presence of detergent. The modified reagent produces a relatively uniform absorbance for six different proteins, and it may be used to determine the protein content of such heterogeneous samples as plasma membranes and mitochondria.

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One-step molybdate method for rapid determination of inorganic phosphate in the presence of protein
โœ Jeanna M. Piper; Stephen J. Lovell ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 435 KB

A rapid method for the estimation of inorganic phosphate in the presence of high concentrations of protein is described. In this procedure protein precipitation is prevented by inclusion either of guanidine hydrochloride or of urea. The assay is based on the observation that the solubilized phosphom