๐”– Bobbio Scriptorium
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A nucleolus-specific phosphoprotein in mouse ascites tumor cells

โœ Scribed by K. Kawashima; M. Izawa

Book ID
104769484
Publisher
Springer
Year
1976
Tongue
English
Weight
375 KB
Volume
3
Category
Article
ISSN
0301-4851

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โœฆ Synopsis

Analysis of proteins in nucleoli and chromatin of mouse ascites tumor cells labeled with [32P]orthophosphate by SDS-polyacrylamide gel electrophoresis showed that a highly radioactive protein was localized in the nucleoli. This protein was purified and the final preparation appeared as a single component on hydroxylapatite column chromatography with or without SDS. This protein was found to be a nucleolus-specific phosphoprotein with a molecular weight of 120 000. The phosphate moiety in this protein turned over very rapidly whereas the protein itself was stable. When the nucleoli were disrupted by EDTA treatment, this unique protein was found as a major protein constituent of the ultracentrifugal supernatant.

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