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A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture

✍ Scribed by Qihui Wang; Xiaoning Mou; Henghua Cao; Qingzhang Meng; Yanni Ma; Pengcheng Han; Junjie Jiang; Hao Zhang; Yue Ma

Book ID
107692187
Publisher
SP Higher Education Press
Year
2012
Tongue
English
Weight
779 KB
Volume
3
Category
Article
ISSN
1674-800X

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## Abstract Ex vivo expansion of limbal stem cells from a small biopsy and its subsequent transplantation is the golden choice of treatment for limbal stem cell deficiency. Use of murine 3T3 feeder layer is a prerequisite for this ex vivo expansion. There is an ever‐increasing demand for feeder fre