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A Novel Technique for Efficient Construction of Large scFv Libraries

โœ Scribed by Weijun Qin; Aizhi Zhao; Yueheng Han; Weihong Wen; Yu Li; Gang Chen; Zhuoli Zhang; He Wang


Book ID
107581539
Publisher
Humana Press Inc
Year
2007
Tongue
English
Weight
257 KB
Volume
37
Category
Article
ISSN
1073-6085

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โœ Heinz D. Osiewacz ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 404 KB

A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15-20 micrograms of