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Construction and utility of 10-kb libraries for efficient clone-gap closure for rice genome sequencing

โœ Scribed by Tae-Jin Yang; Yeisoo Yu; Gyoungju Nah; Michael Atkins; Seunghee Lee; David A. Frisch; Rod A. Wing

Book ID
105813127
Publisher
Springer
Year
2003
Tongue
English
Weight
258 KB
Volume
107
Category
Article
ISSN
0040-5752

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A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15-20 micrograms of