A novel strategy for NMR resonance assignment and protein structure determination
✍ Scribed by Alexander Lemak; Aleksandras Gutmanas; Seth Chitayat; Murthy Karra; Christophe Farès; Maria Sunnerhagen; Cheryl H. Arrowsmith
- Publisher
- Springer Netherlands
- Year
- 2010
- Tongue
- English
- Weight
- 486 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0925-2738
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We present 2D versions of the popular triple resonance HN(CO) CACB, HN(COCA)CACB, HN(CO)CAHA, and HN(COCA) CAHA experiments, commonly used for sequential resonance assignments of proteins. These experiments provide information about correlations between amino proton and nitrogen chemical shifts and
## Abstract Recent advances in NMR techniques to measure anisotropic spin interactions such as residual dipolar coupling (RDC) have provided better insights into protein structure as well as dynamics. Exploitation of RDC, however, still remains challenging because its successful application require