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A novel reporter strain to follow Cre-mediated recombination in T and NK cells

✍ Scribed by Olivier Wurtz; Matthieu Pophillat; Anne-Marie Schmitt-Verhulst; Sylvie Guerder

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
229 KB
Volume
32
Category
Article
ISSN
1526-954X

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✦ Synopsis

Abstract

Summary: The Cre‐loxP system permits the generation of mouse models in which the fate of a cell can be followed through time. Such approach is of great value in immunology because it may allow lineage studies and the dissection of the contribution of specific effector T cells to long‐term memory responses or autoimmune responses. An essential component of such a strategy is the development of appropriate reporter strains of mice in which the inducible reporter molecule is not immunogenic and is well expressed at the cell surface of T cells. We describe here a novel reporter strain of mice that is designed to fulfill these criteria and show that this strain permits the monitoring of Cre‐mediated recombination in both T cells and NK cells. genesis 32:287–292, 2002. © 2002 Wiley‐Liss, Inc.

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