A Novel Reporter GeneMEL1for the Yeast Two-Hybrid System
โ Scribed by Sirpa Aho; Airi Arffman; Tiina Pummi; Jouni Uitto
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 83 KB
- Volume
- 253
- Category
- Article
- ISSN
- 0003-2697
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We describe a simple and efficient one-step method to make cDNA libraries using homologous recombination in yeast. cDNA from any source, together with a linear vector, is used to transform yeast. Through homologous recombination and gap repair, the cDNA is unidirectionally incorporated into the yeas
## Abstract | โโI. | Introduction | 00 | | โII. | The Yeast TwoโHybrid System | 00 | | | A.โโA Brief History | 00 | | | โโโโ1.โโBasis of Yeast TwoโHybrid System | 00 | | | โโโโ2.โโDevelopment of the System | 00 | | | B.โโCurrent Systems | 00 | | | โโโโ1.โโThe GAL4 and LexA Systems | 00 | | |