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A novel, negative selectable marker for gene disruption in Dictyostelium

✍ Scribed by Alastair Morrison; Rolf Marschalek; Theo Dingermann; Adrian J Harwood


Book ID
117522728
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
116 KB
Volume
202
Category
Article
ISSN
0378-1119

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Genetic engineering of Schizosaccharomyc
✍ Grimm, Christian ;Kohli, JΓΌrg ;Murray, Johanne ;Maundrell, Kinsey πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 702 KB

A system is described for gene disruption and replacement in Schizosaccharomyces pombe based on the homologous selectable marker, ura4, the structural gene for orotidine-5'-phosphate decarboxylase. The presence of a single copy of the wild-type gene can rescue a ura4 auxotrophic mutant. Furthermore,