๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A novel nanospray capillary zone electrophoresis/mass spectrometry interface

โœ Scribed by Frank Hsieh; Elizabeth Baronas; Craig Muir; Stephen A. Martin

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
75 KB
Volume
13
Category
Article
ISSN
0951-4198

No coin nor oath required. For personal study only.

โœฆ Synopsis

The high resolution of capillary zone electrophoresis/mass spectrometry (CZE/MS) offers a promising technique to characterize biomolecules in pharmaceutical and biotechnology industries. A novel capillary zone electrophoresis/electrospray ionization time-of-flight mass spectrometry (CZE/ESI-TOF MS) interface was designed in this study to successfully integrate ESI-TOFMS, nanospray, and CZE for biomolecular identification. The interface offers a novel way to take advantage of the high resolution separation achieved during CZE and the detection sensitivity of nanospray ESI-MS. The results showed mixtures of peptides were highly resolved within a few minutes (each CZE electropherogram of a peptide is 2-3 seconds). The novel CZE/ESI-TOFMS interface may simultaneously provide sensitivity, data acquisition speed, mass range, and mass resolution while maintaining resolution of the CZE separation.

๐Ÿ“œ SIMILAR VOLUMES

A sonic spray interface for capillary el
A sonic spray interface for capillary electrophoresis/mass spectrometry
โœ Yukiko Hirabayashi; Atsumu Hirabayashi; Hideaki Koizumi ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 70 KB ๐Ÿ‘ 1 views

We have developed a sonic spray ionization interface that enables coupling of capillary electrophoresis (CE) and mass spectrometry (MS). In sonic spray ionization, a sample solution is sprayed at any solution-flow rate from a sample-introduction capillary with a high-speed gas flow coaxial to the ca

Modified liquid junction interface for n
Modified liquid junction interface for nonaqueous capillary electrophoresis-mass spectrometry
โœ Matti Jussila; Kai Sinervo; Simo P. Porras; Marja-Liisa Riekkola ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 214 KB

## Modified liquid junction interface for nonaqueous capillary electrophoresis-mass spectrometry Electrospray ionization (ESI) is the most widely used ionization method in on-line coupling of capillary electrophoresis-mass spectrometry (CE-MS). The conventional coaxial sheath flow electrospray int

A novel sheathless and electrodeless mic
A novel sheathless and electrodeless microelectrospray interface for the on-line coupling of capillary zone electrophoresis to mass spectrometry
โœ M. Mazereeuw; A.J.P. Hofte; U.R. Tjaden; J. van der Greef ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 206 KB ๐Ÿ‘ 1 views

A novel microelectrospray interface for the on-line coupling of capillary-zone electrophoresis (CZE) and mass spectrometry is described. The outlet of the fused-silica CZE capillary is tapered and serves as the microspray tip. The spray does not contain a sheath flow nor does the capillary tip conta

Disposable Emitters for On-line Capillar
Disposable Emitters for On-line Capillary Zone Electrophoresis/Nanoelectrospray Mass Spectrometry
โœ Kevin P. Bateman; Robert L. White; Pierre Thibault ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 236 KB ๐Ÿ‘ 1 views

A new method for preparing disposable microsprayers, for on-line capillary-zone electrophoresis/electrospray mass spectrometry (CZE/ES-MS) at sub-microliter per minute flow rates, is described. Different means of coupling these microsprayers to the CZE columns are presented, and compared with the mo