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A novel missense mutation 15747 T>C in the mitochondrial cytochrome b gene

✍ Scribed by Isabel Aguilera; José-raúl García-Lozano; Juan Bautista; Y. Campos; J. Arenas; Antonio Núñez-Roldán


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
11 KB
Volume
14
Category
Article
ISSN
1059-7794

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## Detection conditions sequence of primers: F: ACATGGTTAAGGCCTGTTGC R: GCCACATCTGGCTTGAAATT PCR conditions: 3 min at 94oC; 30 cycles of 1 min 94oC, 1 min at 62oC and 1 min at 72oC; 10 min at 72oC electrophoresis: SSCP -10% native polyacrylamide gel (37.5:1 acrylamide: bis-acrylamide), 0.5x TBE,